PolyPhred is available for these platforms:
PolyPhred is available for free to researchers at academic and non-profit institutions. To acquire PolyPhred, please follow this procedure:
Commercial businesses wanting to acquire PolyPhred are required to purchase a commercial license agreement.
To download PolyPhred, click on one of the links below and enter the user ID and password:
For security, the passwords to acquire PolyPhred change weekly. If the
password you have has expired, please request a new one from
Eric Torskey at firstname.lastname@example.org.
If you need to download PolyPhred for more than one operating system, and you got an "authorization failure" message after the first download, please 'reload' this page and try again.
The downloaded file has been 'tarred' and compressed with gzip. It contains:
| ||the executable PolyPhred program *|
| ||a tool for generating fake chromat, PHD and POLY files from sequence data in FASTA format.|
| ||a tool for generating phd and poly files ABI files containing KB base-caller data - an alternative to Phred.|
| ||the PolyPhred documentation in html format.|
| ||a script written in Perl that runs the programs phred and phrap|
To unpack the file, move it to an empty directory. Go to that directory
and run these commands:
tar xzf [filename]where
[filename]is the name of the downloaded file. For example:
tar xzf polyphred_linux.tar.gz
Instructions for installing and setting up PolyPhred are included in the documentation file, polyphred.html.
Download for all platforms (tar/gzip)
Unpack by running:
gunzip -c polyphred-snp-example.tar.gz | tar xvf -
These commands will extract the archive into a directory named
polyphred-snp-example, which contains the
sample data and a README file.
This data set contains an example of heterozygous indel in one of the reads.
Download for all platforms (except DEC-Alpha 4)
After downloading this file, unpack it with the following commands:
gunzip csft2.tar.gz tar xf csft2.tar