How to Acquire PolyPhred

PolyPhred is available for these platforms:
  • Linux (x86 and x86-64)
  • Mac OS X (PowerPC and x86)
  • Cygwin
  • Solaris (x86 and SPARC)
  • SGI IRIX
  • Compaq Tru64 Alpha (formerly DEC OSF/1)
  • HP-UX (PA-RISC)
  • AIX 5 (PowerPC)

Academic and Non-Profit Institutions

PolyPhred is available for free to researchers at academic and non-profit institutions. To acquire PolyPhred, please follow this procedure:

  1. Read the Academic License Agreement, and fill in and submit the request form.

  2. Within a few days, you will receive an email message with a user ID and password that will allow you to download PolyPhred.

  3. When you have the password, click on the appropriate download link below.

Commercial Businesses

Commercial businesses wanting to acquire PolyPhred are required to purchase a commercial license agreement.

  1. Please contact University of Washington TechTransfer Digital Ventures and follow the link "Express Licenses". Specific information about PolyPhred is here.

  2. When we receive confirmation from TechTransfer Digital Ventures, you will receive an email message with a user ID and password that will allow you to download PolyPhred.

  3. When you have the password, click on the appropriate download link below.

Downloading PolyPhred

To download PolyPhred, click on one of the links below and enter the user ID and password:

PolyPhred 6.18

PolyPhred 6.15 Beta

PolyPhred 6.02 Beta

PolyPhred 5.04

For security, the passwords to acquire PolyPhred change weekly. If the password you have has expired, please request a new one from Eric Torskey at polyphred@u.washington.edu.

Instructions

If you need to download PolyPhred for more than one operating system, and you got an "authorization failure" message after the first download, please 'reload' this page and try again.

The downloaded file has been 'tarred' and compressed with gzip. It contains:
   polyphredthe executable PolyPhred program *
   sudophreda tool for generating fake chromat, PHD and POLY files from sequence data in FASTA format.
   polygena tool for generating phd and poly files ABI files containing KB base-caller data - an alternative to Phred.
   polyphred.html   the PolyPhred documentation in html format.
   phredPhrapa script written in Perl that runs the programs phred and phrap

To unpack the file, move it to an empty directory. Go to that directory and run these commands:

  tar xzf [filename]
where [filename] is the name of the downloaded file. For example: 
  tar xzf polyphred_linux.tar.gz

Instructions for installing and setting up PolyPhred are included in the documentation file, polyphred.html.

Example data sets for those who are using PolyPhred for the first time

We suggest that new PolyPhred users also download our example package, which contains a sample data set and a brief tutorial on how to analyze the data with PolyPhred. You do not need a password to download these files.

Download for all platforms (tar/gzip)

Unpack by running: gunzip -c polyphred-snp-example.tar.gz | tar xvf -

These commands will extract the archive into a directory named polyphred-snp-example, which contains the sample data and a README file.

This data set contains an example of heterozygous indel in one of the reads.

Download for all platforms (except DEC-Alpha 4)

After downloading this file, unpack it with the following commands: 

 gunzip csft2.tar.gz
 tar xf csft2.tar

Return to the main PolyPhred page